PUBLICATIONS

2024

• Unterauer, E. M. et al. Spatial proteomics in neurons at single-protein resolution. Cell (2024).
  

• Alsouri, S. et al. Actinin-4 controls survival signaling in B cells by limiting the lateral mobility of B-cell antigen receptors. Eur. J. Immunol. (2024)

• DʼEste, E. et al. Advancing cell biology with nanoscale fluorescence imaging: essential practical considerations. Trends Cell Biol. (2024).
  

2023

• Jong-Bolm, D. de et al. Protein nanobarcodes enable single-step multiplexed fluorescence imaging. PLOS Biol. (2023).

• Alexander, L. T. et al. Protein target highlights in CASP15: Analysis of models by structure providers. Proteins (2023).
  

• Real, K. Q. Z. V. et al. A Versatile Synaptotagmin‐1 Nanobody Provides Perturbation‐Free Live Synaptic Imaging And Low Linkage‐Error in Super‐Resolution Microscopy. Small Methods (2023).

• Saal, K. A. et al. Heat denaturation enables multicolor X10-STED microscopy. Sci Rep 13, 5366 (2023).
  

• Kilisch et al. Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives. Int. J. Mol. Sci. 24(8), 7294 (2023).

• Mougios, N. et al. Trafficking proteins show limited differences in mobility across different postsynaptic spines. iScience 26, 105971 (2023).
  

2022

• Chakrabarti, R. et al. Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses. eLife (2022)

• Iannielli, A. et al. Modeling native and seeded Synuclein aggregation and related cellular dysfunctions in dopaminergic neurons derived by a new set of isogenic iPSC lines with SNCA multiplications. Cell Death Dis (2022).

• Ding, Y. et al. Rapidly Signal‐enhanced Metabolites for Atomic Scale Monitoring of Living Cells with Magnetic Resonance. Chem Methods (2022) 

• Glowacki, S. K. et al. An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry. Chem Commun (2022).

• Oleksiievets, N. et al. Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells. Commun Biology (2022).

2021

• Saul P. et al. Exotic Nuclear Spin Behavior Macromolecules.

  Phys. Chem. Chem. Phys., 2021

• Glowacki S.K. et al. A fluorescent probe for STED microscopy to study NIP-specific B cells. Analyst (2021)

• Kilisch, M. et al. Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer. Biomolecules (2021)

2020

• Mann, F. et al. Quantum defects as a toolbox for covalent carbon nanotube functionalization with peptides and proteins. Angew Chem Int Ed Engl (2020).

• Gerdes, C. et al. A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol. Nat Commun (2020).

• Sograte-Idrissi S. et al. Circumvention of common labelling artefacts using secondary nanobodies. Nanoscale (2020).

• Mamone, S. et al. Singlet-filtered NMR spectroscopy. Sci Adv (2020).

2019

• Götzke, H., et al. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications. Nat Commun (2019) 

• Mann, F. A. et al. Nanobody Conjugated Nanotubes for Targeted Near‐Infrared in vivo Imaging and Sensing. Angew. Chem. (2019).

• Kabatas, S. et al. Fluorinated Nanobodies for Targeted Molecular Imaging of Biological Samples using Nanoscale Secondary Ion Mass Spectrometry. JAAS (2019)

• Callegari, S. et al. A MICOS-TIM22 Association Promotes Carrier Import into Human Mitochondria. J Mol Biol (2019).

• Gomes de Castro, M. A. et al. Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane. Nat Commun (2019).

• Sograte-Idrissi, S. et al. Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors. Cells (2019).
• Kabatas, S. et al. Boron‐Containing Probes for Non‐Optical High‐Resolution Imaging of Biological Samples. Angew. Chem. (2019).

2018

• Maidorn M. Olichon A, Rizzoli SO, Opazo F. (2018) Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons. MAbs
• Mandad S. et al. (2018) The codon sequences predict protein lifetimes and other parameters of the protein life cycle in the mouse brain. Sci Rep
• Fornasiero, E. F. et al. (2018) Precisely measured protein lifetimes in the mouse brain reveal differences across tissues and subcellular fractions. Nat Commun.
• Vanshylla K, Opazo F, Gronke K, Wienands J, Engels N (2018) The extracellular membrane-proximal domain of membrane-bound IgE restricts B cell activation by limiting B cell antigen receptor surface expression. Eur. J. Immunol.

2017

• Gomes de Castro MA, Höbartner C, Opazo F (2017) Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy. PLoS ONE
• Kawabe H, Mitkovski M, Kaeser PS, Hirrlinger J, Opazo F, Nestvogel D, Kalla S, Fejtová A, Verrier SE, Bungers SR, Cooper BH, Varoqueaux F, Wang Y, Nehring RB, Gundelfinger ED, Rosenmund C, Rizzoli SO, Sudhof TC, Rhee J-S, Brose N (2017) ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones. J Cell Biol.

2016

• de Castro MAG, Rammner B, Opazo F (2016) Aptamer Stainings for Super-resolution Microscopy. Methods Mol Biol
• Maidorn M, Rizzoli SO, Opazo F (2016) Tools and limitations to study the molecular composition of synapses by fluorescence microscopy. Biochem J

2015

• Fornasiero EF, Opazo F (2015) Super-resolution imaging for cell biologists: Concepts, applications, current challenges and developments. Bioessays
• Kabatas S, Vreja IC, Saka SK, Höschen C, Kröhnert K, Opazo F, Rizzoli SO, Diederichsen U (2015) A contamination-insensitive probe for imaging specific biomolecules by secondary ion mass spectrometry. Chem Commun (Camb).
• Mikhaylova M, Cloin BMC, Finan K, van den Berg R, Teeuw J, Kijanka MM, Sokolowski M, Katrukha EA, Maidorn M, Opazo F, Moutel S, Vantard M, Perez F, van Bergen En Henegouwen PMP, Hoogenraad CC, Ewers H, Kapitein LC (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun
• Opazo F, Eiden L, Hansen L, Rohrbach F, Wengel J, Kjems J, Mayer G (2015) Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. Mol Ther Nucleic Acids
• Ta H, Keller J, Haltmeier M, Saka SK, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell SW (2015) Mapping molecules in scanning far-field fluorescence nanoscopy. Nat Commun
• Vreja IC, Kabatas S, Saka SK, Kröhnert K, Höschen C, Opazo F, Diederichsen U, Rizzoli SO (2015) Secondary-Ion Mass Spectrometry of Genetically Encoded Targets. Angew Chem Int Ed Engl

2014

• Zhang WI, Röhse H, Rizzoli SO, Opazo F (2014) Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy. Microsc Res Tech

2012

• Opazo F, Levy M, Byrom M, Schäfer C, Geisler C, Groemer TW, Ellington AD, Rizzoli SO (2012) Aptamers as potential tools for superresolution microscopy. Nat Methods
• Wilner SE, Wengerter B, Maier K, de Lourdes Borba Magalhães M, Del Amo DS, Pai S, Opazo F, Rizzoli SO, Yan A, Levy M (2012) An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells. Mol Ther Nucleic Acids

BOOK CHAPTERS

2014

• Felipe Opazo. "Probing Biological Samples in High-Resolution Microscopy: Making Sense of Spots". Super-Resolution Microscopy Techniques in the Neurosciences. Volume 86 of the series Neuromethods pp 369-386. Springer
• Eugenio F. Fornasiero & Felipe Opazo. “Fluorescent Super-Resolution Biology: Cellular and Molecular Applications of Super-resolution Microscopy”. 2014 Advanced Fluorescence Microscopy pp 133 - 152. Elsevier Publisher